Phee401e-mcherry
Web提取T 1 代植株基因组进行PCR鉴定和测序分析，鉴定纯合突变后代的性状表型及激素测定。 结果表明，本实验成功构建了编辑载体pHEE401E- mCherry - CKX3， 拟南芥转化后代中目标基因成功被高效编辑， CKX3 的纯合突变植株表型与野生型差异明显，内源激素含量差异达到显著水平。 为研究 CKX 基因功能奠定了基础。 关键词: 细胞分裂素, 氧化酶, 脱氢酶, 基因 … WebAn mCherry gene under the control of a seed-specific At2S3 promoter was cloned into the plasmid pHEE401E to generate pHEE401E-mCherry14,16. The two gRNA units, which …
Phee401e-mcherry
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WebIn addition, the overexpression construct will be transformed into the Arabidopsis ap1-1 cal-1 mutant plants by floral dipping method. We will also knock out the Arabidopsis … WebApr 10, 2024 · The construction of pHEE401E-CAP1 was described and unpublished work in our lab. Positive transgenic lines were screened on solid Murashige and Skoog (MS) medium containing 20 μg/mL hygromycin and confirmed by sequencing. ... A co-expression assay showed that GFP-CAP1 co-localized with VDAC3-mCherry, a mitochondrial marker (Fig. 5 …
WebHave a question, comment, or need assistance? Send us a message or call (630) 833-0300. Will call available at our Chicago location Mon-Fri 7:00am–6:00pm and Sat … WebVector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 30°C Growth Strain (s) DH10B Copy number High Copy …
WebDownload Table Phenotypic segregation analysis of T2 transgenic lines from publication: Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple ... WebThe pHEE401E_UBQ_Bar vector is a version in which the egg-specific promoter for Cas9 expression in the pHEE401E vector was replaced with the UBQ10 promoter, and the hygromycin resistance gene was ...
WebDec 7, 2024 · 56 usually between 30% and 40% of successful protein expression in E. coli [18]. However, when mCherry 57 is used as a reporter, we observe fluorescence in about 65% of E. coli clones (Supplemental Figure S1). 58 Additionally, a large-scale analysis of the transcription start sites (TSS) of these clones showed a non-
WebChen pHEE401E U6 -26p BsaI yes, cut S. pyogenes Hygro Chen pHDE-35S-Cas9-mCherry-UBQ U6 -26 PmeI yes, cut...opposite strand) mutations. Plasmid Gene/Insert Promoter Selectable Marker PI Publication Nick CRISPR/Cas...homology-directed repair (HDR). lower yalberton farm holiday parkWebMar 21, 2024 · For MIR156B/G and MIR157A/B, the U6-26 promoter-sgRNA or U6-29 promoter-sgRNA cassettes were amplified from pCBC-DT1T2 and cloned into pHEE401E, which harbours a pEC1.2:Cas9 module 24. lower yoder township cambria county paWebJan 1, 2024 · The mCherry-based method provides an efficient and reliable approach to quickly identify transgene-free plants whose genomes have been properly edited. … horror\\u0027s ufWebJan 6, 2024 · Both the first and second constructs were cloned in the pHEE401E vector, which contains a maize codon-optimized Cas9 gene (zCas9) driven by an Arabidopsis egg-cell specific promoter (E.C.1.1) fused with an egg-cell specific enhancer (E.C.1.2) [ 21 ]. Fig. 1 Schematic illustration of gRNA target sites and the two CRISPR multiplexing constructs. a. horror\\u0027s uwWebZepole is your one-stop partner for all of your foodservice needs! From custom kitchen design with equipment delivery and installation; to every day needs like dinnerware, … horror\\u0027s txWebMay 1, 2024 · For fC-0029, the fCas9 fragment was retrieved as Spe I/EcoR I fragments from PHEE401E and inserted into pGreen0029. To construct pGHN-R, the reference gene … horror\\u0027s w1WebJun 22, 2024 · The GFP gene is designed to fuse with part of CRY1 in frame, providing a visual marker for precise insertion of the gene drive element. The mCherry is another … horror\\u0027s w5